Aroid Rooting Medium

A pre-formulated medium designed for the rooting stage of aroid tissue culture.

Formulated with MS nutrients, sucrose and IBA as a convenient starting formulation for the rooting stage.

From shoots to roots.

The rooting stage is the final major stage of the in-vitro propagation workflow before plantlets can be prepared for acclimatization.

Aroid Rooting Medium uses a half-strength MS formulation together with sucrose and IBA (rooting hormone). The result is a dedicated formulation for the rooting stage, without requiring you to prepare the individual components yourself.

Formulation

Each litre of prepared medium contains:

The formulation is supplied as a dry powder.

Agar is not included. [Agar→]

When should I use Rooting Medium?

Use Rooting Medium after the multiplication stage when suitable shoots or plantlets are ready to enter the rooting stage.

Why pre-formulated?

Preparing a tissue culture medium from individual ingredients requires accurate measurement of several components.
Aroid Rooting Medium simplifies this process by combining the formulation into one convenient powder.

One formulation. Consistent preparation. Less measuring.

What You'll Need

To make sure you succeed with tissue culture propagation, you will need the following equipment and materials:

Gelling agent: Agar or other gelling agent such as Geltrite .

Sterile workspace

• Still Air Box (SAB) or Laminar Flow Hood
• 70% Isopropyl Alcohol (IPA) for surface disinfection

Vessels & sealing

• Culture jars, tubes, or Magenta boxes (pre-sterilized)
• Breathable caps or Parafilm for sealing

Plant material

• Healthy aroid explant (nodal segment, shoot tip, or leaf section)
• Surface sterilization agents: 70% ethanol, 10–20% bleach solution, sterile distilled water

Equipment

• Scalpel with sterile blades
• Forceps / tweezers
• Sterile gloves and face mask
• Pressure cooker or autoclave (for sterilizing tools and vessels)
• pH meter and pH adjustment solutions (KOH / HCl)

The InVitro Garden workflow

01 — ESTABLISH
Establishment Medium
↓
02 — MULTIPLY
Multiplication Medium
↓
03 — ROOT
Rooting Medium
↓
04 — ACCLIMATIZE

Preparation

Recipe for 1 liter finished solution:

1. Measure 22.2g of rooting media (34.5g for Establishment and Multiplication media).
2. Add 800ml of distilled/deionized water, dissolve thoroughly
   
3. Adjust pH to 5.6-5.8 pH. Use pH meter or strips.
4. Slowly add 7-9g of agar if a solid medium is desired, heat to dissolve.
5. Fill the remaining 200ml for a total volume of 1L medium.
6. When fully dissolved, pour in to culture jars and sterilize the prepared medium using an autoclave/pressure cooker.
7. Allow the medium to cool before introducing plant material.

This recipe is based on achieving 1 liter of finished media solution. If less/more is desired, make sure to adjust all ingredients accordingly.
Always follow the preparation instructions supplied with the product.

Aroid Medium Series

Explore the complete Aroid Media Set→

Important

Keep out of reach of children.
Avoid inhaling powder and contact with eyes.
For plant tissue culture and horticultural use only.
Not intended for human or animal consumption.