Aroid Medium Set - 3-Stage Kit

All three Aroid Mediums in one complete set.

Includes a package of each Aroid Establishment, Multiplication and Rooting Medium — providing a complete three-stage foundation for aroid tissue culture.

The complete Aroid Media Series.

Plant tissue culture often involves different stages, each with different requirements.

The InVitro Garden Aroid Medium Set brings together all three formulations in the Aroid Media Series, giving you a convenient starting point for working through the complete in-vitro propagation workflow.

01 — ESTABLISH
Introduce and establish suitable plant material in culture.
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02 — MULTIPLY
Transfer established cultures to a multiplication-oriented medium.
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03 — ROOT
Move suitable shoots or plantlets to the rooting medium.
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04—ACCLIMATIZE
Prepare rooted plantlets for transition out of culture.

One set. Three stages.

Designed for Aroids

The formulations are designed as general starting formulations for commonly propagated aroids, including:

Monstera · Philodendron · Alocasia · Anthurium · Epipremnum

Plant response can vary depending on species, cultivar, explant type and culture conditions.

The formulations should therefore be considered general-purpose starting formulations rather than species-specific protocols.

What's included?

· Aroid Establishment Medium

An MS-based medium formulated as a general starting formulation for the establishment stage of aroid tissue culture.

MS + vitamins · Sucrose · BAP

· Aroid Multiplication Medium

A pre-formulated medium designed for the multiplication stage of established cultures.

MS + vitamins · Sucrose · BAP · NAA

· Aroid Rooting Medium

A half-strength MS-based formulation designed as a general starting point for the rooting stage.

½-strength MS + vitamins · Sucrose · IBA

What You'll Need

To make sure you succeed with tissue culture propagation, you will need the following equipment and materials:

Gelling agent: Agar or other gelling agent such as Geltrite .

Sterile workspace

• Still Air Box (SAB) or Laminar Flow Hood
• 70% Isopropyl Alcohol (IPA) for surface disinfection

Vessels & sealing

• Culture jars, tubes, or Magenta boxes (pre-sterilized)
• Breathable caps or Parafilm for sealing

Plant material

• Healthy aroid explant (nodal segment, shoot tip, or leaf section)
• Surface sterilization agents: 70% ethanol, 10–20% bleach solution, sterile distilled water

Equipment

• Scalpel with sterile blades
• Forceps / tweezers
• Sterile gloves and face mask
• Pressure cooker or autoclave (for sterilizing tools and vessels)
• pH meter and pH adjustment solutions (KOH / HCl)

Preparation 

Recipe for 1 liter finished solution:

1. Measure 34.5g of aroid media. (22.2g for rooting medium)
2. Add 800ml of distilled/deionized water, dissolve thoroughly
   
3. Adjust pH to 5.6-5.8 pH, use pH buffer for higher/lower pH. (pH meter recommended)
4. Slowly add 7-9g of agar if a solid medium is desired, heat to dissolve.
5. Fill the remaining 200ml for a total volume of 1L medium.
6. When fully dissolved, pour in to culture jars and sterilize the prepared medium using an autoclave/pressure cooker.
7. Allow the medium to cool before introducing plant material.

This recipe is based on achieving 1 liter of finished media solution. If less/more is desired, make sure to adjust all ingredients accordingly. 
Always follow the preparation instructions supplied with the product.

Agar is not included. [Agar→]

Important

Keep out of reach of children.
Avoid inhaling powder and contact with eyes.
For plant tissue culture and horticultural use only.
Not intended for human or animal consumption.