{"product_id":"tissue-culture-medium-set-invitro-garden","title":"Aroid Medium Set - 3-Stage Kit","description":"\u003cp\u003e\u003cstrong\u003eAll three Aroid Mediums in one complete set.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003c\/strong\u003eIncludes a package of each \u003cstrong\u003eAroid Establishment, Multiplication and Rooting Medium\u003c\/strong\u003e — providing a complete three-stage foundation for aroid tissue culture.\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eThe complete Aroid Media Series.\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003ePlant tissue culture often involves different stages, each with different requirements.\u003c\/p\u003e\n\u003cp\u003eThe \u003cstrong\u003eInVitro Garden Aroid Medium Set\u003c\/strong\u003e brings together all three formulations in the Aroid Media Series, giving you a convenient starting point for working through the complete in-vitro propagation workflow.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e01 — ESTABLISH\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cspan role=\"text\"\u003eIntroduce and establish suitable plant material in culture.\u003c\/span\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e\u003cbr\u003e\u003cspan\u003e↓\u003c\/span\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e02 — MULTIPLY\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cspan role=\"text\"\u003eTransfer established cultures to a multiplication-oriented medium.\u003c\/span\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e\u003cbr\u003e\u003cspan\u003e↓\u003c\/span\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e03 — ROOT\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003eMove suitable shoots or plantlets to the rooting medium.\u003cbr\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cspan role=\"text\"\u003e\u003cstrong\u003e\u003cspan\u003e↓\u003cbr\u003e\u003c\/span\u003e04—ACCLIMATIZE\u003cbr\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003ePrepare rooted plantlets for transition out of culture.\u003c\/p\u003e\n\u003ch3 style=\"text-align: center;\"\u003e\u003cstrong\u003eOne set. Three stages.\u003c\/strong\u003e\u003c\/h3\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eDesigned for Aroids\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe formulations are designed as general starting formulations for commonly propagated aroids, including:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMonstera · Philodendron · Alocasia · Anthurium · Epipremnum\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePlant response can vary depending on species, cultivar, explant type and culture conditions.\u003c\/p\u003e\n\u003cp\u003eThe formulations should therefore be considered \u003cstrong\u003egeneral-purpose starting formulations rather than species-specific protocols.\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eWhat's included?\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003e\u003cstrong\u003e· Aroid Establishment Medium\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eAn MS-based medium formulated as a general starting formulation for the establishment stage of aroid tissue culture.\u003c\/p\u003e\n\u003cp\u003eMS + vitamins · Sucrose · BAP\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e· Aroid Multiplication Medium\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA pre-formulated medium designed for the multiplication stage of established cultures.\u003c\/p\u003e\n\u003cp\u003eMS + vitamins · Sucrose · BAP · NAA\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e· Aroid Rooting Medium\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003eA half-strength MS-based formulation designed as a general starting point for the rooting stage.\u003c\/p\u003e\n\u003cp\u003e½-strength MS + vitamins · Sucrose · IBA\u003c\/p\u003e\n\u003ch2 class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eWhat You'll Need\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cspan\u003eTo make sure you succeed with tissue culture propagation, you will need the following equipment and materials:\u003c\/span\u003e\u003cstrong\u003e\u003cspan\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eGelling agent: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003eAgar\u003c\/strong\u003e or other gelling agent such as \u003cstrong\u003eGeltrite\u003c\/strong\u003e .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eSterile workspace\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eStill Air Box (SAB) or Laminar Flow Hood\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e70% Isopropyl Alcohol (IPA) for surface disinfection\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eVessels \u0026amp; sealing\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eCulture jars, tubes, or Magenta boxes (pre-sterilized)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBreathable caps or Parafilm for sealing\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003ePlant material\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eHealthy aroid explant (nodal segment, shoot tip, or leaf section)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSurface sterilization agents: 70% ethanol, 10–20% bleach solution, sterile distilled water\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eEquipment\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eScalpel with sterile blades\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eForceps \/ tweezers\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSterile gloves and face mask\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePressure cooker or autoclave (for sterilizing tools and vessels)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003epH meter and pH adjustment solutions (KOH \/ HCl)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003ePreparation\u003c\/strong\u003e \u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eRecipe for 1 liter finished solution:\u003cbr\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eMeasure 34.5g of aroid media. (22.2g for rooting medium)\u003c\/li\u003e\n\u003cli\u003eAdd 800ml of distilled\/deionized water, dissolve thoroughly\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAdjust pH to 5.6-5.8 pH, use pH buffer for higher\/lower pH. (pH meter recommended)\u003c\/li\u003e\n\u003cli\u003eSlowly add 7-9g of agar if a solid medium is desired, heat to dissolve.\u003c\/li\u003e\n\u003cli\u003eFill the remaining 200ml for a total volume of 1L medium.\u003c\/li\u003e\n\u003cli\u003eWhen fully dissolved, pour in to culture jars and sterilize the prepared medium using an autoclave\/pressure cooker.\u003c\/li\u003e\n\u003cli\u003eAllow the medium to cool before introducing plant material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThis recipe is based on achieving 1 liter of finished media solution. If less\/more is desired, make sure to adjust all ingredients accordingly. \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eAlways follow the preparation instructions supplied with the product.\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cspan style=\"font-size: 0.875rem;\"\u003eAgar is not included. [\u003c\/span\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/agar\" title=\"Agar | InVitro Garden\" rel=\"noopener\" style=\"color: rgb(64, 64, 64);\" target=\"_blank\"\u003e\u003cspan\u003e\u003cstrong\u003eAgar→]\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003c\/span\u003e\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch3 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003e\u003c\/strong\u003e\u003cbr\u003e\n\u003c\/h3\u003e\n\u003ch3 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eImportant\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eKeep out of reach of children.\u003cbr\u003eAvoid inhaling powder and contact with eyes.\u003cbr\u003eFor plant tissue culture and horticultural use only.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eNot intended for human or animal consumption.\u003c\/p\u003e\n\u003cp\u003e\u003cbr\u003e\u003c\/p\u003e","brand":"InVitro Garden","offers":[{"title":"1L x 3","offer_id":56198836257153,"sku":"KIT-TCM-1Lx3","price":349.0,"currency_code":"SEK","in_stock":false},{"title":"5L x 3","offer_id":56198836289921,"sku":"KIT-TCM-5Lx3","price":1099.0,"currency_code":"SEK","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0957\/9112\/0769\/files\/ChatGPTImage24sep.202609_40_57_5.png?v=1790235694","url":"https:\/\/invitrogarden.com\/it\/products\/tissue-culture-medium-set-invitro-garden","provider":"InVitro Garden","version":"1.0","type":"link"}