{"product_id":"tissue-culture-establishment-medium","title":"Aroid Establishment Medium","description":"\u003cp class=\"PDq2pG_selectionAnchorContainer\"\u003e\u003cmeta charset=\"UTF-8\"\u003e\u003cstrong\u003eA pre-formulated medium designed for the establishment stage of aroid tissue culture.\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp class=\"PDq2pG_selectionAnchorContainer\"\u003eDeveloped as a convenient starting formulation for establishing aroid explants in vitro, with MS nutrients, vitamins, sucrose, and BAP already combined in to one ready-to-use formulation.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eEstablish your cultures.\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe establishment stage is the first step in the in-vitro propagation process.\u003cbr\u003e\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAroid Establishment Medium\u003c\/strong\u003e provides a pre-formulated nutritional and hormonal environment designed as a general starting point for establishing aroid plant material in culture.\u003c\/p\u003e\n\u003cp\u003eInstead of measuring individual components such as basal salts, vitamins, sucrose and BAP yourself, everything is combined into a single powder formulation.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003eMeasure → Dissolve → Prepare → Sterilize → Culture\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eFormulation\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eEach litre of prepared medium contains:\u003c\/p\u003e\n\u003cdiv class=\"group TyagGW_tableContainer\"\u003e\n\u003cdiv class=\"TyagGW_tableWrapper flex flex-col-reverse w-fit\" tabindex=\"-1\"\u003e\n\u003ctable class=\"w-fit min-w-(--thread-content-width)\"\u003e\n\u003cthead\u003e\n\u003ctr\u003e\n\u003cth class=\"last:pe-10\" style=\"text-align: left;\"\u003eComponent\u003c\/th\u003e\n\u003cth class=\"last:pe-10\" style=\"text-align: left;\"\u003eConcentration\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd\u003eMS + vitamins\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e4.4 g\/L\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eSucrose\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e30 g\/L\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003ctr\u003e\n\u003ctd\u003eBAP\u003c\/td\u003e\n\u003ctd\u003e\u003cstrong\u003e1.0 mg\/L\u003c\/strong\u003e\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003c\/div\u003e\n\u003c\/div\u003e\n\u003cp\u003eThe formulation is supplied as a dry powder.\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eAgar is not included.\u003cbr\u003e\u003c\/strong\u003eAgar can be purchased separately and added when preparing a solid medium.\u003ca href=\"https:\/\/invitrogarden.com\/products\/tissue-culture-agar\" title=\"Tissue Culture Agar | InVitro Garden\" rel=\"noopener\" target=\"_blank\"\u003e\u003cspan style=\"text-decoration: underline; color: rgb(64, 64, 64);\"\u003e\u003cstrong\u003e Agar→\u003c\/strong\u003e\u003c\/span\u003e\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2\u003e\n\u003cstrong\u003eDesigned for Aroids\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eThe formulation is designed as a general starting medium for a range of commonly propagated aroids, including:\u003c\/p\u003e\n\u003cp\u003e\u003cstrong\u003eMonstera · Philodendron · Alocasia · Anthurium · Epipremnum\u003c\/strong\u003e\u003c\/p\u003e\n\u003cp\u003ePlant response can vary between species, cultivars, explant types and culture conditions. This formulation should therefore be considered a \u003cstrong\u003egeneral starting formulation\u003c\/strong\u003e, rather than a species-specific formula.\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003eWhen should I use Establishment Medium?\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eEstablishment Medium is intended for the \u003cstrong\u003einitial establishment stage\u003c\/strong\u003e of your tissue culture workflow.\u003cbr\u003e\u003cbr\u003eOnce cultures have successfully established and sufficient new growth has developed, they can be transferred to a multiplication-oriented formulation.\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/multiplication-media\" title=\"Tissue Culture Multiplication Medium | InVitro Garden\" style=\"color: rgb(64, 64, 64);\" rel=\"noopener\" target=\"_blank\"\u003e\u003cstrong\u003e Aroid Multiplication Medium\u003cspan style=\"text-decoration: underline;\"\u003e\u003c\/span\u003e\u003c\/strong\u003e\u003cstrong\u003e→\u003c\/strong\u003e\u003c\/a\u003e\u003cbr\u003e\u003cbr\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003ch2 class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eWhat You'll Need\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cspan\u003eTo make sure you succeed with tissue culture propagation, you will need the following equipment and materials:\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eGelling agent: \u003c\/span\u003e\u003c\/strong\u003e\u003cspan\u003e\u003cstrong\u003eAgar\u003c\/strong\u003e or other gelling agent such as \u003cstrong\u003eGeltrite\u003c\/strong\u003e .\u003c\/span\u003e\u003c\/p\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eSterile workspace\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eStill Air Box (SAB) or Laminar Flow Hood\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003e70% Isopropyl Alcohol (IPA) for surface disinfection\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eVessels \u0026amp; sealing\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eCulture jars, tubes, or Magenta boxes (pre-sterilized)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eBreathable caps or Parafilm for sealing\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003ePlant material\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eHealthy aroid explant (nodal segment, shoot tip, or leaf section)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eSurface sterilization agents: 70% ethanol, 10–20% bleach solution, sterile distilled water\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003cp class=\"_p_1x4zg_107\"\u003e\u003cstrong\u003e\u003cspan\u003eEquipment\u003c\/span\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003cul class=\"_ul_1x4zg_146\"\u003e\n\u003cli\u003e\u003cspan\u003eScalpel with sterile blades\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003eForceps \/ tweezers\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\n\u003cspan\u003eSterile gloves and face mask\u003c\/span\u003e\u003cspan\u003e\u003c\/span\u003e\n\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003ePressure cooker or autoclave (for sterilizing tools and vessels)\u003c\/span\u003e\u003c\/li\u003e\n\u003cli\u003e\u003cspan\u003epH meter and pH adjustment solutions (KOH \/ HCl)\u003c\/span\u003e\u003c\/li\u003e\n\u003c\/ul\u003e\n\u003ch3\u003e\u003cstrong\u003e\u003cbr\u003eThe complete InVitro Garden workflow\u003c\/strong\u003e\u003c\/h3\u003e\n\u003cp\u003e\u003cstrong\u003e01 — ESTABLISH\u003c\/strong\u003e\u003cbr\u003eAroid Establishment Medium\u003cbr\u003e↓\u003cbr\u003e\u003cstrong\u003e02 — MULTIPLY\u003c\/strong\u003e\u003cbr\u003eAroid Multiplication Medium\u003cbr\u003e↓\u003cbr\u003e\u003cstrong\u003e03 — ROOT\u003c\/strong\u003e\u003cbr\u003eAroid Rooting Medium\u003cbr\u003e\u003cbr\u003e\u003c\/p\u003e\n\u003ch2 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003ePreparation\u003c\/strong\u003e \u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h2\u003e\n\u003cp\u003eRecipe for 1 liter Aroid Medium:\u003cbr\u003e\u003c\/p\u003e\n\u003col\u003e\n\u003cli\u003eMeasure 34.5g of aroid media. (22.2g for rooting medium)\u003c\/li\u003e\n\u003cli\u003eAdd 800ml of distilled\/deionized water, dissolve thoroughly\u003cbr\u003e\n\u003c\/li\u003e\n\u003cli\u003eAdjust pH to 5.6-5.8, use pH buffer for higher\/lower pH. (recommend having a pH meter)\u003c\/li\u003e\n\u003cli\u003eSlowly add 6-8g of agar if a solid medium is desired, heat to dissolve.\u003c\/li\u003e\n\u003cli\u003eFill the remaining 200ml for a total volume of 1L medium.\u003c\/li\u003e\n\u003cli\u003eWhen fully dissolved, pour in to culture jars and sterilize the prepared medium using an autoclave\/pressure cooker.\u003c\/li\u003e\n\u003cli\u003eAllow the medium to cool before introducing plant material.\u003c\/li\u003e\n\u003c\/ol\u003e\n\u003cp\u003eThis recipe is based on achieving 1 liter of finished media solution. If less\/more is desired, make sure to adjust all ingredients accordingly. \u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003eAlways follow the preparation instructions supplied with the product.\u003cbr\u003e\u003cbr\u003e\u003c\/strong\u003e\u003cstrong\u003e\u003c\/strong\u003e\u003c\/p\u003e\n\u003ch2\u003e\u003cstrong\u003e\u003cspan role=\"text\"\u003eAroid Medium Series\u003c\/span\u003e\u003c\/strong\u003e\u003c\/h2\u003e\n\u003ctable style=\"width: 100%; height: 19px;\"\u003e\n\u003cthead\u003e\n\u003ctr style=\"height: 19px;\"\u003e\n\u003cth class=\"last:pe-10\" style=\"width: 26.42487%; text-align: left; height: 19px;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003eStage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/th\u003e\n\u003cth class=\"last:pe-10\" style=\"width: 35.578584%; text-align: left; height: 19px;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003eMedium\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/th\u003e\n\u003cth class=\"last:pe-10\" style=\"width: 36.442142%; text-align: left; height: 19px;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003ePurpose\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/th\u003e\n\u003c\/tr\u003e\n\u003c\/thead\u003e\n\u003c\/table\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.42487%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003cstrong\u003e01\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.578584%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/establishment-media\" title=\"Aroid Establishment Medium | InVitro Garden\" rel=\"noopener\" style=\"color: rgb(64, 64, 64);\" target=\"_blank\"\u003eESTABLISHMENT\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.614853%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003eInitial establishment\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"width: 100%;\"\u003e\n\u003ctbody\u003e\n\u003ctr\u003e\n\u003ctd style=\"width: 26.42487%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003cstrong\u003e02\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.693626%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/multiplication-media\" title=\"Aroid Multiplication Medium | InVitro Garden\" rel=\"noopener\" style=\"color: rgb(64, 64, 64);\" target=\"_blank\"\u003eMULTIPLICATION\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.499811%;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003eMultiplication stage\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ctable style=\"width: 100%; height: 35px;\"\u003e\n\u003ctbody\u003e\n\u003ctr style=\"height: 35px;\"\u003e\n\u003ctd style=\"width: 26.42487%; height: 35px;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003cstrong\u003e03\u003c\/strong\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 35.751295%; height: 35px;\"\u003e\n\u003cp\u003e\u003cspan style=\"color: rgb(64, 64, 64);\"\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/rooting-media\" title=\"Aroid Rooting Medium | InVitro Garden\" rel=\"noopener\" style=\"color: rgb(64, 64, 64);\" target=\"_blank\"\u003eROOTING\u003c\/a\u003e\u003c\/span\u003e\u003c\/p\u003e\n\u003c\/td\u003e\n\u003ctd style=\"width: 36.442142%; height: 35px;\"\u003e\n\u003cp\u003eRoot development\u003c\/p\u003e\n\u003c\/td\u003e\n\u003c\/tr\u003e\n\u003c\/tbody\u003e\n\u003c\/table\u003e\n\u003ch3 class=\"PDq2pG_selectionAnchorContainer\"\u003e\u003cspan style=\"color: rgb(5, 0, 0);\"\u003e\u003cstrong\u003e\u003ca href=\"https:\/\/invitrogarden.com\/products\/aroid-medium-set-invitro-garden\" title=\"Aroid Medium Set | InVitro Garden\" style=\"color: rgb(5, 0, 0);\" rel=\"noopener\" target=\"_blank\"\u003eExplore the complete Aroid Media Set→\u003c\/a\u003e\u003c\/strong\u003e\u003c\/span\u003e\u003c\/h3\u003e\n\u003ch3 class=\"PDq2pG_selectionAnchorContainer\"\u003e\n\u003cstrong\u003e\u003cbr\u003eImportant\u003c\/strong\u003e\u003cspan class=\"PDq2pG_selectionAnchor\"\u003e\u003c\/span\u003e\n\u003c\/h3\u003e\n\u003cp\u003eKeep out of reach of children.\u003cbr\u003eAvoid inhaling powder and contact with eyes.\u003cbr\u003eFor plant tissue culture and horticultural use only.\u003cstrong\u003e\u003cbr\u003e\u003c\/strong\u003eNot intended for human or animal consumption.\u003c\/p\u003e","brand":"InVitro Garden","offers":[{"title":"1L","offer_id":56189121954177,"sku":"TCM-AEM-1L","price":119.0,"currency_code":"SEK","in_stock":false},{"title":"5L","offer_id":56189121986945,"sku":"TCM-AEM-5L","price":449.0,"currency_code":"SEK","in_stock":false},{"title":"10","offer_id":56195614376321,"sku":"TCM-AEM-10L","price":749.0,"currency_code":"SEK","in_stock":false}],"thumbnail_url":"\/\/cdn.shopify.com\/s\/files\/1\/0957\/9112\/0769\/files\/ChatGPT_Image_24_sep._2026_09_40_57_4.png?v=1790235737","url":"https:\/\/invitrogarden.com\/fi\/products\/tissue-culture-establishment-medium","provider":"InVitro Garden","version":"1.0","type":"link"}